JC virus DNA in healthy brain tissue: a challenge for progressive multifocal leukoencephalopathy diagnosis
Brains from 10 patients aged 68 to 96 years at time of death were studied for JC viral DNA
and common papovaviral capsid protein. In situ hybridization of JC viral DNA was performed
by affinity cytochemistry using a biotinylated fragment of JC viral DNA.
Immunohistochemistry was performed on brain tissue by the avidin DH-biotinylated
horseradish peroxidase technique using polyclonal antibody raised against the papovaviral
capsid protein. Viral protein and DNA were detected in 4 of 10 patients. JC virus may be …
and common papovaviral capsid protein. In situ hybridization of JC viral DNA was performed
by affinity cytochemistry using a biotinylated fragment of JC viral DNA.
Immunohistochemistry was performed on brain tissue by the avidin DH-biotinylated
horseradish peroxidase technique using polyclonal antibody raised against the papovaviral
capsid protein. Viral protein and DNA were detected in 4 of 10 patients. JC virus may be …
Brains from 10 patients aged 68 to 96 years at time of death were studied for JC viral DNA and common papovaviral capsid protein. In situ hybridization of JC viral DNA was performed by affinity cytochemistry using a biotinylated fragment of JC viral DNA. Immunohistochemistry was performed on brain tissue by the avidin DH-biotinylated horseradish peroxidase technique using polyclonal antibody raised against the papovaviral capsid protein. Viral protein and DNA were detected in 4 of 10 patients. JC virus may be present in the brains of aged patients more frequently than previously suspected.
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