Pseudoparticle neutralization is a reliable assay to measure immunity and cross-reactivity to H5N1 influenza viruses
I Alberini, E Del Tordello, A Fasolo, NJ Temperton… - Vaccine, 2009 - Elsevier
I Alberini, E Del Tordello, A Fasolo, NJ Temperton, G Galli, C Gentile, E Montomoli…
Vaccine, 2009•ElsevierThe standard serological methods present limitations for the measurement of immunity
against H5N1 influenza strains. The hemagglutination inhibition (HI) assay lacks sensitivity
and requires standardization, while the viral micro-neutralization (MN) assay needs
handling of live virus. We produced pseudoparticles expressing hemagglutinin from clades
1 or 2 H5N1 in order to measure neutralizing antibodies in human sera after prime-boost
vaccination with plain or MF59-adjuvanted H5N1 clade 1 subunit vaccines. Titers measured …
against H5N1 influenza strains. The hemagglutination inhibition (HI) assay lacks sensitivity
and requires standardization, while the viral micro-neutralization (MN) assay needs
handling of live virus. We produced pseudoparticles expressing hemagglutinin from clades
1 or 2 H5N1 in order to measure neutralizing antibodies in human sera after prime-boost
vaccination with plain or MF59-adjuvanted H5N1 clade 1 subunit vaccines. Titers measured …
The standard serological methods present limitations for the measurement of immunity against H5N1 influenza strains. The hemagglutination inhibition (HI) assay lacks sensitivity and requires standardization, while the viral micro-neutralization (MN) assay needs handling of live virus. We produced pseudoparticles expressing hemagglutinin from clades 1 or 2 H5N1 in order to measure neutralizing antibodies in human sera after prime-boost vaccination with plain or MF59-adjuvanted H5N1 clade 1 subunit vaccines. Titers measured by pseudoparticles neutralization (PPN) assay significantly correlated with those measured by HI, single radial haemolysis or MN, with a PPN titer of 1:357 corresponding to an MN titer of 1:80. Notably, results from the PPN assay, confirm that MF59-H5N1 vaccine induces potent and long-lasting neutralizing antibody responses not only against the vaccine strain, but also against several heterologous clade 2 strains. Overall, the PPN assay represents a valid alternative to conventional serological methods for the evaluation of H5N1 vaccine immunogenicity.
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