Structures of the sugar chains of rabbit immunoglobin G: occurrence of asparagine-linked sugar chains in Fab fragment
T Taniguchi, T Mizuochi, M Beale, RA Dwek… - Biochemistry, 1985 - ACS Publications
T Taniguchi, T Mizuochi, M Beale, RA Dwek, TW Rademacher, A Kobata
Biochemistry, 1985•ACS PublicationsMaterials and Methods Chemicals and Enzymes. NaB3H4 (347.8 mCi/mmol) was
purchased from New England Nuclear, Boston, MA. NaB2H4 (98%) was purchased from
Merck, Co., Darmstadt, Germany.-Mannosidase, ß-galactosidase, and dA-acetylhexos-1
Abbreviations: Tris-HCl, tris (hydroxymethyl) aminomethane hydrochloride; EDTA,
ethylenediaminetetraacetic acid; SDS-PAGE, so-dium dodecyl sulfate-polyacrylamide gel
electrophoresis; Gal, galactose; GlcNAc,/V-acetylglucosamine; Man, mannose; Fuc, fucose …
purchased from New England Nuclear, Boston, MA. NaB2H4 (98%) was purchased from
Merck, Co., Darmstadt, Germany.-Mannosidase, ß-galactosidase, and dA-acetylhexos-1
Abbreviations: Tris-HCl, tris (hydroxymethyl) aminomethane hydrochloride; EDTA,
ethylenediaminetetraacetic acid; SDS-PAGE, so-dium dodecyl sulfate-polyacrylamide gel
electrophoresis; Gal, galactose; GlcNAc,/V-acetylglucosamine; Man, mannose; Fuc, fucose …
Materials and Methods
Chemicals and Enzymes. NaB3H4 (347.8 mCi/mmol) was purchased from New England Nuclear, Boston, MA. NaB2H4 (98%) was purchased from Merck, Co., Darmstadt, Germany.-Mannosidase, ß-galactosidase, and dA-acetylhexos-1 Abbreviations: Tris-HCl, tris (hydroxymethyl) aminomethane hydrochloride; EDTA, ethylenediaminetetraacetic acid; SDS-PAGE, so-dium dodecyl sulfate-polyacrylamide gel electrophoresis; Gal, galactose; GlcNAc,/V-acetylglucosamine; Man, mannose; Fuc, fucose. All sugars mentioned in this paper were of the d configuration except for fucose, which had the l configuration. Subscript OT is used to indicate NaB3H4-reduced oligosaccharides.
ACS Publications