The spatial relationship between Ca2+ channels and Ca2+-activated channels and the function of Ca2+-buffering in avian sensory neurons
In order to learn about the endogenous Ca2+-buffering in the cytoplasm of chick dorsal root
ganglion (DRG) neurons and the distance separating the ryanodine receptor Ca2+ release
channels (RyRs) from the plasma membrane, we monitored the amplitude and time course
of Ca2+-activated Cl–currents (lCl (Ca)) in protocols that manipulated Ca2+-buffering. lCl
(Ca) was activated by Ca2+ influx via voltage-gated Ca2+ channels or by Ca2+ release via
RyRs activated by 10mM caffeine. lCl (Ca) was measured in neurons at 20° C and 35° C …
ganglion (DRG) neurons and the distance separating the ryanodine receptor Ca2+ release
channels (RyRs) from the plasma membrane, we monitored the amplitude and time course
of Ca2+-activated Cl–currents (lCl (Ca)) in protocols that manipulated Ca2+-buffering. lCl
(Ca) was activated by Ca2+ influx via voltage-gated Ca2+ channels or by Ca2+ release via
RyRs activated by 10mM caffeine. lCl (Ca) was measured in neurons at 20° C and 35° C …