Spatiotemporal Dynamics of Inositol 1,4,5-Trisphosphate That Underlies Complex Ca2+ Mobilization Patterns
Science, 1999•science.org
Inositol 1, 4, 5-trisphosphate (IP3) is a second messenger that elicits complex
spatiotemporal patterns of calcium ion (Ca2+) mobilization and has essential roles in the
regulation of many cellular functions. In Madin-Darby canine kidney epithelial cells, green
fluorescent protein–tagged pleckstrin homology domain translocated from the plasma
membrane to the cytoplasm in response to increased concentration of IP3. The detection of
translocation enabled monitoring of IP3 concentration changes within single cells and …
spatiotemporal patterns of calcium ion (Ca2+) mobilization and has essential roles in the
regulation of many cellular functions. In Madin-Darby canine kidney epithelial cells, green
fluorescent protein–tagged pleckstrin homology domain translocated from the plasma
membrane to the cytoplasm in response to increased concentration of IP3. The detection of
translocation enabled monitoring of IP3 concentration changes within single cells and …
Inositol 1,4,5-trisphosphate (IP3) is a second messenger that elicits complex spatiotemporal patterns of calcium ion (Ca2+) mobilization and has essential roles in the regulation of many cellular functions. In Madin-Darby canine kidney epithelial cells, green fluorescent protein–tagged pleckstrin homology domain translocated from the plasma membrane to the cytoplasm in response to increased concentration of IP3. The detection of translocation enabled monitoring of IP3 concentration changes within single cells and revealed spatiotemporal dynamics in the concentration of IP3 synchronous with Ca2+ oscillations and intracellular and intercellular IP3 waves that accompanied Ca2+ waves. Such changes in IP3 concentration may be fundamental to Ca2+ signaling.
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