[HTML][HTML] Inhibition of cell growth by conditional expression of kpm, a human homologue of Drosophila warts/lats tumor suppressor
Y Kamikubo, A Takaori-Kondo, T Uchiyama… - Journal of Biological …, 2003 - ASBMB
kpm is a human serine/threonine kinase that is homologous to Drosophila tumor suppressor
warts/lats and its mammalian homologue LATS1. In order to define the biological function of
kpm, we generated stable transfectants of wild-type kpm (kpm-wt), a kinase-dead mutant
ofkpm (kpm-kd), and luciferase in HeLa Tet-Off cells under the tetracycline-responsive
promoter. Western blot analysis showed that high levels of expression of kpm-wt as well as
kpm-kd with an apparent mass of 150 kDa were induced after the removal of doxycycline …
warts/lats and its mammalian homologue LATS1. In order to define the biological function of
kpm, we generated stable transfectants of wild-type kpm (kpm-wt), a kinase-dead mutant
ofkpm (kpm-kd), and luciferase in HeLa Tet-Off cells under the tetracycline-responsive
promoter. Western blot analysis showed that high levels of expression of kpm-wt as well as
kpm-kd with an apparent mass of 150 kDa were induced after the removal of doxycycline …