Ascorbic acid and dehydroascorbic acid measurements in human plasma and serum
KR Dhariwal, WO Hartzell, M Levine - The American journal of clinical …, 1991 - Elsevier
We investigated whether circulating ascorbic acid in humans is protein bound or free and
whether ascorbic acid exists in its reduced form alone as ascorbic acid or in its reduced and
oxidized forms as ascorbic acid and dehydroascorbic acid, respectively. Ascorbic acid and
dehydroascorbic acid were determined by using HPLC with coulometric electrochemical
detection, and protein binding was determined by centrifugal ultrafiltration. Ascorbic acid
was free in plasma and serum of normal, healthy volunteers, 10 men and 10 women …
whether ascorbic acid exists in its reduced form alone as ascorbic acid or in its reduced and
oxidized forms as ascorbic acid and dehydroascorbic acid, respectively. Ascorbic acid and
dehydroascorbic acid were determined by using HPLC with coulometric electrochemical
detection, and protein binding was determined by centrifugal ultrafiltration. Ascorbic acid
was free in plasma and serum of normal, healthy volunteers, 10 men and 10 women …