Electrofusion of mouse blastomeres
JZ Kubiak, AK Tarkowski - Experimental cell research, 1985 - Elsevier
JZ Kubiak, AK Tarkowski
Experimental cell research, 1985•ElsevierFusion of blastomeres of 2-cell mouse embryos with an intact zona pellucida can be induced
with electric pulses. Fusion was most frequent with the field strength of 1 kV/cm and direct
current pulses of 100–250 μsec duration. An electrolyte solution (PBS) can be used instead
of a non-electrolyte solution (0.3 M mannitol). The viability of blastomeres fused in these two
types of solution is similar. Fused 2-cell blastomeres develop into tetraploid blastocysts but
die after implantation. Embryos in which blastomeres failed to fuse despite the treatment …
with electric pulses. Fusion was most frequent with the field strength of 1 kV/cm and direct
current pulses of 100–250 μsec duration. An electrolyte solution (PBS) can be used instead
of a non-electrolyte solution (0.3 M mannitol). The viability of blastomeres fused in these two
types of solution is similar. Fused 2-cell blastomeres develop into tetraploid blastocysts but
die after implantation. Embryos in which blastomeres failed to fuse despite the treatment …
Abstract
Fusion of blastomeres of 2-cell mouse embryos with an intact zona pellucida can be induced with electric pulses. Fusion was most frequent with the field strength of 1 kV/cm and direct current pulses of 100–250 μsec duration. An electrolyte solution (PBS) can be used instead of a non-electrolyte solution (0.3 M mannitol). The viability of blastomeres fused in these two types of solution is similar. Fused 2-cell blastomeres develop into tetraploid blastocysts but die after implantation. Embryos in which blastomeres failed to fuse despite the treatment (diploid controls) can develop till term. The technique can also be applied to 3- and 4-cell embryos and to zona-free oocytes and blastomeres.
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