Isolation of low-frequency class-switch variants from rat hybrid myelomas
G Hale, SP Cobbold, H Waldmann, G Easter… - Journal of …, 1987 - Elsevier
Class-switch variants have been isolated from rat-rat hybrid myelomas by sib selection using
a simple assay based on red cell-labelled antiglobulins. The variants detected are
consistent with the gene order deduced from molecular cloning. They appear to arise
spontaneously at a rate approximately ten-fold lower than for mouse cell lines but the rate of
switching back to the parental isotype is substantial in comparison. An IgG2b variant
antibody having the same specificity as CAMPATH-1 for human lymphocytes and monocytes …
a simple assay based on red cell-labelled antiglobulins. The variants detected are
consistent with the gene order deduced from molecular cloning. They appear to arise
spontaneously at a rate approximately ten-fold lower than for mouse cell lines but the rate of
switching back to the parental isotype is substantial in comparison. An IgG2b variant
antibody having the same specificity as CAMPATH-1 for human lymphocytes and monocytes …