High‐throughput generation of tagged stable cell lines for proteomic analysis
JZ Torres, JJ Miller, PK Jackson - Proteomics, 2009 - Wiley Online Library
JZ Torres, JJ Miller, PK Jackson
Proteomics, 2009•Wiley Online LibraryWe present an optimized system for rapid generation of localization and affinity purification‐
tagged mammalian stable cell lines that facilitates complex purification and interacting
protein identification. The improved components of this method, including the flexibility of
inducible expression, circumvent issues associated with toxicity, clonal selection, sample
yields and time to data acquisition. We have applied this method to the study of cell‐cycle
regulators and novel microtubule‐associated proteins.
tagged mammalian stable cell lines that facilitates complex purification and interacting
protein identification. The improved components of this method, including the flexibility of
inducible expression, circumvent issues associated with toxicity, clonal selection, sample
yields and time to data acquisition. We have applied this method to the study of cell‐cycle
regulators and novel microtubule‐associated proteins.
Abstract
We present an optimized system for rapid generation of localization and affinity purification‐tagged mammalian stable cell lines that facilitates complex purification and interacting protein identification. The improved components of this method, including the flexibility of inducible expression, circumvent issues associated with toxicity, clonal selection, sample yields and time to data acquisition. We have applied this method to the study of cell‐cycle regulators and novel microtubule‐associated proteins.
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