[CITATION][C] Simplified column liquid chromatographic method for measuring urinary oxalate
BC McWhinney, DM Cowley, AH Chalmers - Journal of Chromatography B …, 1986 - Elsevier
Urines (24 h) for analysis were collected either over 50 ml of 5 M hydrochloric acid or both
0.2-0.6 mmol sodium thimerosal and 20-25 mmol disodium EDTA to prevent ascorbate
instability and oxalate precipitation and generation [191. A Z-ml urine sample and 2 ml DAB
reagent were mixed in a l&ml polystyrene tube, capped and heated in a domestic pressure
cooker at 125 C for 15 min. The pressure cooker was allowed to cool to about 70-8O” C, and
the tubes were removed and allowed to cool to room temperature. The pH of each sample …
0.2-0.6 mmol sodium thimerosal and 20-25 mmol disodium EDTA to prevent ascorbate
instability and oxalate precipitation and generation [191. A Z-ml urine sample and 2 ml DAB
reagent were mixed in a l&ml polystyrene tube, capped and heated in a domestic pressure
cooker at 125 C for 15 min. The pressure cooker was allowed to cool to about 70-8O” C, and
the tubes were removed and allowed to cool to room temperature. The pH of each sample …