[PDF][PDF] PKC pathway and ERK/MAPK pathway are required for induction of cyclin D1 and p21Waf1 during 12-o-tetradecanoylphorbol 13-acetate-induced …
E Matsumoto, M Hatanaka, M Bohgaki… - Kobe Journal of …, 2006 - researchgate.net
E Matsumoto, M Hatanaka, M Bohgaki, S Maeda
Kobe Journal of Medical Sciences, 2006•researchgate.netMATERIALS AND METHODS Cell culture. Human myeloleukemia cell lines, HL60
(JCRB0085) and U937 (JCRB9021), were obtained from Health Science Research
Resources Bank, and THP-1 (RCB1189) and KG-1 (RCB1166) from RIKEN BioResource
Center. All of them differentiate into monocytes/macrophages by TPA treatment. Cells were
maintained in RPMI 1640 medium (GIBCO) supplemented with 10% fetal bovine serum (ICN
Biomedicals) at 37 C in a humidified atmosphere consisting of 5% CO2. 12-o …
(JCRB0085) and U937 (JCRB9021), were obtained from Health Science Research
Resources Bank, and THP-1 (RCB1189) and KG-1 (RCB1166) from RIKEN BioResource
Center. All of them differentiate into monocytes/macrophages by TPA treatment. Cells were
maintained in RPMI 1640 medium (GIBCO) supplemented with 10% fetal bovine serum (ICN
Biomedicals) at 37 C in a humidified atmosphere consisting of 5% CO2. 12-o …
MATERIALS AND METHODS
Cell culture. Human myeloleukemia cell lines, HL60 (JCRB0085) and U937 (JCRB9021), were obtained from Health Science Research Resources Bank, and THP-1 (RCB1189) and KG-1 (RCB1166) from RIKEN BioResource Center. All of them differentiate into monocytes/macrophages by TPA treatment. Cells were maintained in RPMI 1640 medium (GIBCO) supplemented with 10% fetal bovine serum (ICN Biomedicals) at 37 C in a humidified atmosphere consisting of 5% CO2. 12-o-tetradecanoylphorbol 13-acetate (Wako) dissolved in DMSO (Wako) at 1 μg/μL was added to 2× 105 cells/mL culture media at a final concentration of 30 ng/mL. Cycloheximide (Wako) dissolved in RPMI1640 and bisindolylmaleimide I, Gö6976,
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