A method for the fractionation of the high-mobility-group non-histone chromosomal proteins
C Sanders - Biochemical and Biophysical Research …, 1977 - Elsevier
C Sanders
Biochemical and Biophysical Research Communications, 1977•ElsevierA method is given for the preparation of four non-histone chromosomal proteins, one of
which, protein 14, hitherto has not been isolated. The method also enables the preparation
of histone H1 in gram quantities. The four non-histone chromosomal proteins so prepared
are all polar molecules over 50% of each being composed of acidic and basic amino acids.
It is also shown that protein 14 can be prepared from calf thymus without prior isolation of
chromatin.
which, protein 14, hitherto has not been isolated. The method also enables the preparation
of histone H1 in gram quantities. The four non-histone chromosomal proteins so prepared
are all polar molecules over 50% of each being composed of acidic and basic amino acids.
It is also shown that protein 14 can be prepared from calf thymus without prior isolation of
chromatin.
Abstract
A method is given for the preparation of four non-histone chromosomal proteins, one of which, protein 14, hitherto has not been isolated. The method also enables the preparation of histone H1 in gram quantities. The four non-histone chromosomal proteins so prepared are all polar molecules over 50% of each being composed of acidic and basic amino acids. It is also shown that protein 14 can be prepared from calf thymus without prior isolation of chromatin.
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