[HTML][HTML] Human apolipoprotein B transgenic mice generated with 207-and 145-kilobase pair bacterial artificial chromosomes: evidence that a distant 5′-element …
LB Nielsen, SPA McCormick, V Pierotti, C Tam… - Journal of Biological …, 1997 - ASBMB
We reported previously that∼ 80-kilobase pair (kb) P1 bacteriophage clones spanning
either the human or mouse apoB gene (clones p158 and p649, respectively) confer apoB
expression in the liver of transgenic mice, but not in the intestine. We hypothesized that the
absence of intestinal expression was due to the fact that these clones lacked a distant DNA
element controlling intestinal expression. To test this possibility, transgenic mice were
generated with 145-and 207-kb bacterial artificial chromosomes (BACs) that contained the …
either the human or mouse apoB gene (clones p158 and p649, respectively) confer apoB
expression in the liver of transgenic mice, but not in the intestine. We hypothesized that the
absence of intestinal expression was due to the fact that these clones lacked a distant DNA
element controlling intestinal expression. To test this possibility, transgenic mice were
generated with 145-and 207-kb bacterial artificial chromosomes (BACs) that contained the …