Long‐term measurement of cerebral blood flow and metabolism in a rat chronic hypoperfusion model
T Otori, T Katsumata, H Muramatsu… - Clinical and …, 2003 - Wiley Online Library
T Otori, T Katsumata, H Muramatsu, F Kashiwagi, Y Katayama, A Terashi
Clinical and Experimental Pharmacology and Physiology, 2003•Wiley Online LibraryRat bilateral common carotid artery occlusion (BCAO) was used as a chronic cerebral
hypoperfusion model. We observed autoradiographically the long‐term changes in regional
cerebral blood flow (rCBF) and regional cerebral glucose utilization (rCGU) after 2 days and
1, 4 and 8 weeks of BCAO and in controls. Regions evaluated included the cerebral cortex,
white matter and basal ganglia. Pathological changes were also observed with Klüver–
Barrera and haematoxylin–eosin staining. 2. After 2 days, rCBF was significantly reduced to …
hypoperfusion model. We observed autoradiographically the long‐term changes in regional
cerebral blood flow (rCBF) and regional cerebral glucose utilization (rCGU) after 2 days and
1, 4 and 8 weeks of BCAO and in controls. Regions evaluated included the cerebral cortex,
white matter and basal ganglia. Pathological changes were also observed with Klüver–
Barrera and haematoxylin–eosin staining. 2. After 2 days, rCBF was significantly reduced to …
Summary
1. Rat bilateral common carotid artery occlusion (BCAO) was used as a chronic cerebral hypoperfusion model. We observed autoradiographically the long‐term changes in regional cerebral blood flow (rCBF) and regional cerebral glucose utilization (rCGU) after 2 days and 1, 4 and 8 weeks of BCAO and in controls. Regions evaluated included the cerebral cortex, white matter and basal ganglia. Pathological changes were also observed with Klüver–Barrera and haematoxylin–eosin staining.
2. After 2 days, rCBF was significantly reduced to 33–58% in the cortex, white matter and amygdala and similar reductions were observed after 1 week.
3. After 4 weeks, rCBF recovered; however, rCBF remained significantly reduced in the occipital cortex, white matter, globus pallidus and substantia nigra.
4. After 2 days, rCGU was mostly maintained but, after 1 week, rCGU was reduced significantly to 40–70% in the cortex, white matter, basal ganglia and thalamus. Four weeks later, these reductions were no longer seen.
5. Rarefaction of the white matter was observed from 1 week.
6. These results showed that the BCAO in rats is an appropriate model for chronic cerebral hypoperfusion and that uncoupling of rCBF and rCGU was observed from 2 days until 4 weeks in the white matter.
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