[HTML][HTML] Rheb binds tuberous sclerosis complex 2 (TSC2) and promotes S6 kinase activation in a rapamycin-and farnesylation-dependent manner
AF Castro, JF Rebhun, GJ Clark, LA Quilliam - Journal of Biological …, 2003 - ASBMB
Recently the tuberous sclerosis complex 2 (TSC2) tumor suppressor gene product has been
identified as a negative regulator of protein synthesis upstream of the mTOR and ribosomal
S6 kinases. Because of the homology of TSC2 with GTPase-activating proteins for Rap1, we
examined whether a Ras/Rap-related GTPase might be involved in this process. TSC2 was
found to bind to Rheb-GTP in vitro and to reduce Rheb GTP levels in vivo. Over-expression
of Rheb but not Rap1 promoted the activation of S6 kinase in a rapamycin-dependent …
identified as a negative regulator of protein synthesis upstream of the mTOR and ribosomal
S6 kinases. Because of the homology of TSC2 with GTPase-activating proteins for Rap1, we
examined whether a Ras/Rap-related GTPase might be involved in this process. TSC2 was
found to bind to Rheb-GTP in vitro and to reduce Rheb GTP levels in vivo. Over-expression
of Rheb but not Rap1 promoted the activation of S6 kinase in a rapamycin-dependent …