OBJECTIVE: The absence of effective strategies for the ex vivo expansion of human
hematopoietic stem cells (HSCs) limits the development of many cell-based therapies. Prior
attempts to stimulate HSC expansion have focused on media supplementation using
cytokines and growth factors. In these cultures, cellular and microenvironmental
compositions change with time. In this study, the impact of controlling these dynamic
changes on HSC output is determined. MATERIALS AND METHODS: Cord blood–derived …