Publisher Summary This chapter discusses the three genes whose overall targeting
frequencies suggest that homologous recombination is a broadly applicable technique in
human ES cells. The homologous recombination frequencies are roughly comparable to
those observed for mouse ES cells and suggest that, although successful transfection
strategies differ between human and mouse ES cells, homologous recombination itself may
be similar. Homologous recombination in human ES cells will be important for studying gene …