Generation of mouse-induced pluripotent stem cells by transient expression of a single nonviral polycistronic vector

F Gonzalez, M Barragan Monasterio… - Proceedings of the …, 2009 - National Acad Sciences
F Gonzalez, M Barragan Monasterio, G Tiscornia, N Montserrat Pulido, R Vassena…
Proceedings of the National Academy of Sciences, 2009National Acad Sciences
Induced pluripotent stem (iPS) cells have generated keen interest due to their potential use
in regenerative medicine. They have been obtained from various cell types of both mice and
humans by exogenous delivery of different combinations of Oct4, Sox2, Klf4, c-Myc, Nanog,
and Lin28. The delivery of these transcription factors has mostly entailed the use of
integrating viral vectors (retroviruses or lentiviruses), carrying the risk of both insertional
mutagenesis and oncogenesis due to misexpression of these exogenous factors. Therefore …
Induced pluripotent stem (iPS) cells have generated keen interest due to their potential use in regenerative medicine. They have been obtained from various cell types of both mice and humans by exogenous delivery of different combinations of Oct4, Sox2, Klf4, c-Myc, Nanog, and Lin28. The delivery of these transcription factors has mostly entailed the use of integrating viral vectors (retroviruses or lentiviruses), carrying the risk of both insertional mutagenesis and oncogenesis due to misexpression of these exogenous factors. Therefore, obtaining iPS cells that do not carry integrated transgene sequences is an important prerequisite for their eventual therapeutic use. Here we report the generation of iPS cell lines from mouse embryonic fibroblasts with no evidence of integration of the reprogramming vector in their genome, achieved by nucleofection of a polycistronic construct coexpressing Oct4, Sox2, Klf4, and c-Myc.
National Acad Sciences