Induction of pluripotent stem cells by defined factors is greatly improved by small-molecule compounds
Nature biotechnology, 2008•nature.com
Reprogramming of mouse and human somatic cells can be achieved by ectopic expression
of transcription factors, but with low efficiencies. We report that DNA methyltransferase and
histone deacetylase (HDAC) inhibitors improve reprogramming efficiency. In particular,
valproic acid (VPA), an HDAC inhibitor, improves reprogramming efficiency by more than
100-fold, using Oct4-GFP as a reporter. VPA also enables efficient induction of pluripotent
stem cells without introduction of the oncogene c-Myc.
of transcription factors, but with low efficiencies. We report that DNA methyltransferase and
histone deacetylase (HDAC) inhibitors improve reprogramming efficiency. In particular,
valproic acid (VPA), an HDAC inhibitor, improves reprogramming efficiency by more than
100-fold, using Oct4-GFP as a reporter. VPA also enables efficient induction of pluripotent
stem cells without introduction of the oncogene c-Myc.
Abstract
Reprogramming of mouse and human somatic cells can be achieved by ectopic expression of transcription factors, but with low efficiencies. We report that DNA methyltransferase and histone deacetylase (HDAC) inhibitors improve reprogramming efficiency. In particular, valproic acid (VPA), an HDAC inhibitor, improves reprogramming efficiency by more than 100-fold, using Oct4-GFP as a reporter. VPA also enables efficient induction of pluripotent stem cells without introduction of the oncogene c-Myc.
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