Detection and activation of human Vα24+ natural killer T cells using α-galactosyl ceramide-pulsed dendritic cells
S Fujii, K Shimizu, RM Steinman… - Journal of immunological …, 2003 - Elsevier
Human CD1d-restricted natural killer T (NKT) cells, which are postulated to regulate the
immune response in several clinical settings, can be activated by α-galactosylceramide (α-
GalCer) presented by CD1d molecules on antigen presenting cells (APCs). Simple methods
to quantify NKT function in fresh blood will greatly benefit studies targeting NKT cells in
humans. Here we show that freshly isolated human NKT cells can be readily quantified by
an enzyme-linked immunospot (ELISPOT) assay and have a Th1 profile (secreting interferon …
immune response in several clinical settings, can be activated by α-galactosylceramide (α-
GalCer) presented by CD1d molecules on antigen presenting cells (APCs). Simple methods
to quantify NKT function in fresh blood will greatly benefit studies targeting NKT cells in
humans. Here we show that freshly isolated human NKT cells can be readily quantified by
an enzyme-linked immunospot (ELISPOT) assay and have a Th1 profile (secreting interferon …