The renal outer medullary potassium channel (ROMK) of the thick ascending limb (TAL) is a
critical component of the counter-current multiplication mechanism. In this study, two new
antibodies raised to ROMK were used to investigate changes in the renal abundance of
ROMK with treatments known to strongly promote TAL function. These antibodies
specifically recognized protein of the predicted size of 45 kD in immunoblots of rat kidney or
COS cells transfected with ROMK cDNA. Infusion of 1-deamino-(8-D-arginine)-vasopressin …