[HTML][HTML] WNK4 diverts the thiazide-sensitive NaCl cotransporter to the lysosome and stimulates AP-3 interaction
AR Subramanya, J Liu, DH Ellison, JB Wade… - Journal of biological …, 2009 - ASBMB
With-no-lysine kinase 4 (WNK4) inhibits electroneutral sodium chloride reabsorption by
attenuating the cell surface expression of the thiazide-sensitive NaCl cotransporter (NCC).
The underlying mechanism for this effect remains poorly understood. Here, we explore how
WNK4 affects the trafficking of NCC through its interactions with intracellular sorting
machinery. An analysis of NCC cell surface lifetime showed that WNK4 did not alter the net
rate of cotransporter internalization. In contrast, direct measurements of forward trafficking …
attenuating the cell surface expression of the thiazide-sensitive NaCl cotransporter (NCC).
The underlying mechanism for this effect remains poorly understood. Here, we explore how
WNK4 affects the trafficking of NCC through its interactions with intracellular sorting
machinery. An analysis of NCC cell surface lifetime showed that WNK4 did not alter the net
rate of cotransporter internalization. In contrast, direct measurements of forward trafficking …