Method for quantitation of IgG subclass antibodies in mouse serum by enzyme-linked immunosorbent assay
RK Gupta, GR Siber - Journal of immunological methods, 1995 - Elsevier
One of the methods for calibration of antigen specific antibodies in a serum involves parallel
titration of known concentrations of purified normal immunoglobulins (Igs) and a specific
antiserum. We evaluated the effect of three methods for capturing known concentrations of
purified normal mouse Igs (IgG, IgG subclasses and IgM) on the anti-tetanus toxoid antibody
concentrations assigned to a reference mouse serum:(1) direct coating,(2) capture by pre-
coated anti-mouse IgG (Fc) specific antibodies, and (3) capture by pre-coated anti-mouse …
titration of known concentrations of purified normal immunoglobulins (Igs) and a specific
antiserum. We evaluated the effect of three methods for capturing known concentrations of
purified normal mouse Igs (IgG, IgG subclasses and IgM) on the anti-tetanus toxoid antibody
concentrations assigned to a reference mouse serum:(1) direct coating,(2) capture by pre-
coated anti-mouse IgG (Fc) specific antibodies, and (3) capture by pre-coated anti-mouse …