Many nuclear hormone receptors are involved in the regulation of skin homeostasis. However, their role in the epithelial compartment of the skin in stress situations, such as skin healing, has not been addressed yet. The healing of a skin wound after an injury involves three major cell types: immune cells, which are recruited to the wound bed; dermal fibroblasts; and epidermal and hair follicle keratinocytes. Our previous studies have revealed important but nonredundant roles of PPARα and β/δ in the reparation of the skin after a mechanical injury in the adult mouse. However, the mesenchymal or epithelial cellular compartment in which PPARα and β/δ play a role could not be determined in the null mice used, which have a germ line PPAR gene invalidation. In the present work, the role of PPARα was studied in keratinocytes, using transgenic mice that express a PPARα mutant with dominant-negative (dn) activity specifically in keratinocytes. This dn PPARα lacks the last 13 C terminus amino acids, binds to a PPARα agonist, but is unable to release the nuclear receptor corepressor and to recruit the coactivator p300. When selectively expressed in keratinocytes of transgenic mice, dn PPARαΔ13 causes a delay in the healing of skin wounds, accompanied by an exacerbated inflammation. This phenotype, which is similar to that observed in PPARα null mice, strongly suggests that during skin healing, PPARα is required in keratinocytes rather than in other cell types.