[PDF][PDF] Agarose infiltration improves morphology of cryostat sections of lung
AC Halbower, RJ Mason, SH Abman… - Laboratory …, 1994 - researchgate.net
BACKGROUND: Optimal morphology and immunohistochemistry of the lung requires the
organ to remain fully distended. When the lung is prepared for frozen sections, especially
during the stage of cryoprotection, liquid fixatives leak out and the lung tends to collapse. We
sought to develop a new technique to improve the morphology of frozen sections of the lung
while facilitating 1 tissue handling and immunoreactivity.| EXPERIMENTAL DESIGN:
Human, fetal sheep and adult rat lungs were distended with 1% low| temperature melting …
organ to remain fully distended. When the lung is prepared for frozen sections, especially
during the stage of cryoprotection, liquid fixatives leak out and the lung tends to collapse. We
sought to develop a new technique to improve the morphology of frozen sections of the lung
while facilitating 1 tissue handling and immunoreactivity.| EXPERIMENTAL DESIGN:
Human, fetal sheep and adult rat lungs were distended with 1% low| temperature melting …