Isolation and characterization of the mouse cardiac myosin heavy chain genes
J Gulick, A Subramaniam, J Neumann… - Journal of Biological …, 1991 - ASBMB
Two mouse genomic libraries were probed in order to isolate the murine cardiac myosin
heavy chain (MHC) genes. Two overlapping cosmid clones that encode the cardiac genes
were isolated. One of them encompasses the entire alpha-cardiac MHC gene, its 5'-flanking
region and approximately 10 kilobase pairs (kb) of the 3'-end of the beta-cardiac MHC gene
which we determined is located approximately 4 kb upstream of the alpha-cardiac MHC
gene. Four clones isolated from a bacteriophage library were found to overlap with the 5' …
heavy chain (MHC) genes. Two overlapping cosmid clones that encode the cardiac genes
were isolated. One of them encompasses the entire alpha-cardiac MHC gene, its 5'-flanking
region and approximately 10 kilobase pairs (kb) of the 3'-end of the beta-cardiac MHC gene
which we determined is located approximately 4 kb upstream of the alpha-cardiac MHC
gene. Four clones isolated from a bacteriophage library were found to overlap with the 5' …