[HTML][HTML] Stable isotope labeling by amino acids in cell culture (SILAC) and proteome quantitation of mouse embryonic stem cells to a depth of 5,111 proteins
J Graumann, NC Hubner, JB Kim, K Ko, M Moser… - Molecular & Cellular …, 2008 - ASBMB
Embryonic stem (ES) cells are pluripotent cells isolated from mammalian preimplantation
embryos. They are capable of differentiating into all cell types and therefore hold great
promise in regenerative medicine. Here we show that murine ES cells can be fully SILAC
(stable isotope labeling by amino acids in cell culture)-labeled when grown feeder-free
during the last phase of cell culture. We fractionated the SILAC-labeled ES cell proteome by
one-dimensional gel electrophoresis and by isoelectric focusing of peptides. High resolution …
embryos. They are capable of differentiating into all cell types and therefore hold great
promise in regenerative medicine. Here we show that murine ES cells can be fully SILAC
(stable isotope labeling by amino acids in cell culture)-labeled when grown feeder-free
during the last phase of cell culture. We fractionated the SILAC-labeled ES cell proteome by
one-dimensional gel electrophoresis and by isoelectric focusing of peptides. High resolution …