Graded increase in probability of eosinophilic differentiation of HL-60 promyelocytic leukemia cells induced by culture under alkaline conditions
SA Fischkoff - Leukemia research, 1988 - Elsevier
HL-60 cells differentiate primarily to eosinophils instead of neutrophils when cultured with
butyric acid if they have previously been cultured under alkaline conditions (pH 7.6). To
determine the nature of the process by which this occurs, a group of single-cell derived
clones was produced from HL-60 cells after prolonged passage under alkaline conditions.
When these clones were induced to mature with butyric acid, each clone demonstrated a
characteristic proportion of mature eosinophils and neutrophils. This property was stable for …
butyric acid if they have previously been cultured under alkaline conditions (pH 7.6). To
determine the nature of the process by which this occurs, a group of single-cell derived
clones was produced from HL-60 cells after prolonged passage under alkaline conditions.
When these clones were induced to mature with butyric acid, each clone demonstrated a
characteristic proportion of mature eosinophils and neutrophils. This property was stable for …