Dynamitin is a commonly used inhibitor of cytoplasmic dynein-based motility in living cells.
Dynamitin does not inhibit dynein directly but instead acts by causing disassembly of
dynactin, a multiprotein complex required for dynein-based movement. In dynactin,
dynamitin is closely associated with the subunits p150 Glued and p24, which together form
the shoulder and projecting arm structures of the dynactin molecule. In this study, we explore
the way in which exogenous dynamitin effects dynactin disruption. We find that pure …