Affinity selection from biological libraries

JF Smothers, S Henikoff, P Carter - Science, 2002 - science.org
Science, 2002science.org
Phage architecture and phage display.(Left) A protein (black coil) is shown fused on its
carboxy-terminal end to the phage minor coat protein pIII (yellow) of a filamentous phage.
Peptides and proteins have also been displayed by genetic fusion to the other minor coat
proteins [pVI (purple), pVII (red), and pIX (green)] or to the major coat protein [pVIII
(blue)].(Right) Key steps in the selection of interactors from phage display libraries.
Phage architecture and phage display.(Left) A protein (black coil) is shown fused on its carboxy-terminal end to the phage minor coat protein pIII (yellow) of a filamentous phage. Peptides and proteins have also been displayed by genetic fusion to the other minor coat proteins [pVI (purple), pVII (red), and pIX (green)] or to the major coat protein [pVIII (blue)].(Right) Key steps in the selection of interactors from phage display libraries.
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