Improved purification of leukocidin fromStaphylococcus aureus and toxin distribution among hospital strains
V Finck-Barbançon, G Pre, Y Pie - Research in microbiology, 1991 - Elsevier
For purification of F and S components of the Panton-Valentine leukocidin, an easy three-
step method using fast protein liquid chromatography was developed to replace the time-
consuming purification procedures previously published. This technique enabled the
recovery of 13 and 17 mg of purified F and S, respectively, per litre of culture supernatant.
Affinity-purified neutralizing polyclonal antibodies were obtained against each individual
component. One hundred and thirty-nineStaphylococcus aureus strains isolated from …
step method using fast protein liquid chromatography was developed to replace the time-
consuming purification procedures previously published. This technique enabled the
recovery of 13 and 17 mg of purified F and S, respectively, per litre of culture supernatant.
Affinity-purified neutralizing polyclonal antibodies were obtained against each individual
component. One hundred and thirty-nineStaphylococcus aureus strains isolated from …