Adenoviral-mediated delivery of a viral chemokine binding protein blocks CC-chemokine activity in vitro and in vivo
CA Bursill, S Cai, KM Channon, DR Greaves - Immunobiology, 2003 - Elsevier
Chemokines are important mediators of leukocyte recruitment and activation that play critical
roles in the pathology of inflammatory diseases such as atherosclerosis, rheumatoid arthritis
and asthma. The vaccinia virus (strain Lister) expresses a 35 kDa soluble protein ('35K') that
binds and inactivates a wide range of CC chemokines. We generated a recombinant
adenovirus encoding soluble 35K (Ad35K). Ad35K-infected cell culture medium, containing
recombinant 35K, potently reduced migration of CCR5-transfected 293 cells by 95% in …
roles in the pathology of inflammatory diseases such as atherosclerosis, rheumatoid arthritis
and asthma. The vaccinia virus (strain Lister) expresses a 35 kDa soluble protein ('35K') that
binds and inactivates a wide range of CC chemokines. We generated a recombinant
adenovirus encoding soluble 35K (Ad35K). Ad35K-infected cell culture medium, containing
recombinant 35K, potently reduced migration of CCR5-transfected 293 cells by 95% in …