[PDF][PDF] Differential roles of TLR2 and TLR4 in recognition of gram-negative and gram-positive bacterial cell wall components

O Takeuchi, K Hoshino, T Kawai, H Sanjo, H Takada… - Immunity, 1999 - cell.com
O Takeuchi, K Hoshino, T Kawai, H Sanjo, H Takada, T Ogawa, K Takeda, S Akira
Immunity, 1999cell.com
Abstract Toll-like receptor (TLR) 2 and TLR4 are implicated in the recognition of various
bacterial cell wall components, such as lipopolysaccharide (LPS). To investigate in vivo
roles of TLR2, we generated TLR2-deficient mice. In contrast to LPS unresponsiveness in
TLR4-deficient mice, TLR2-deficient mice responded to LPS to the same extent as wild-type
mice. TLR2-deficient macrophages were hyporesponsive to several Gram-positive bacterial
cell walls as well as Staphylococcus aureus peptidoglycan. TLR4-deficient macrophages …
Abstract
Toll-like receptor (TLR) 2 and TLR4 are implicated in the recognition of various bacterial cell wall components, such as lipopolysaccharide (LPS). To investigate in vivo roles of TLR2, we generated TLR2-deficient mice. In contrast to LPS unresponsiveness in TLR4-deficient mice, TLR2-deficient mice responded to LPS to the same extent as wild-type mice. TLR2-deficient macrophages were hyporesponsive to several Gram-positive bacterial cell walls as well as Staphylococcus aureus peptidoglycan. TLR4-deficient macrophages lacked the response to Gram-positive lipoteichoic acids. These results demonstrate that TLR2 and TLR4 recognize different bacterial cell wall components in vivo and TLR2 plays a major role in Gram-positive bacterial recognition.
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