[HTML][HTML] Cloning and characterization of HuR, a ubiquitously expressed Elav-like protein
WJ Ma, S Cheng, C Campbell, A Wright… - Journal of Biological …, 1996 - ASBMB
The neuronal-specific Elav-like proteins (HuD, Hel-N, and HuC) contain three RNP-type
concensus motifs and bind to AU-rich elements. We have identified and cloned a fourth
member of this family (HuR) that is expressed in a wide variety of cell types. The purified
recombinant protein binds avidly to the AU-rich element in c-fos and interleukin-3 mRNAs. In
the case of the c-fos AU-rich element, HuR binds to a core element of 27 nucleotides that
contain AUUUA, AUUUUA, and AUUUUUA motifs. Mutational analysis has shown that all …
concensus motifs and bind to AU-rich elements. We have identified and cloned a fourth
member of this family (HuR) that is expressed in a wide variety of cell types. The purified
recombinant protein binds avidly to the AU-rich element in c-fos and interleukin-3 mRNAs. In
the case of the c-fos AU-rich element, HuR binds to a core element of 27 nucleotides that
contain AUUUA, AUUUUA, and AUUUUUA motifs. Mutational analysis has shown that all …