The intermediate filament systems in the eye lens

M Der Perng, A Sandilands, J Kuszak, R Dahm… - Methods in cell …, 2004 - Elsevier
Publisher Summary This chapter describes the basic methods to purify important lens
fractions and to track the distribution of IF proteins in the eye lens and their associated
proteins. It also provides the description of the methods used for the preparation, sectioning,
and examination of eye lenses. The IF proteins of the lens help in determining its optical
properties. Whereas, a transparent lens is normal, a lens with either a reduced level of
transparency or a localized opacity is abnormal and/or pathological. One of the pitfalls in the …