Assignment of human desmin gene to band 2q35 by nonradioactive in situ hybridization
E Viegas-Péquignot, LZ Lin, B Dutrillaux, F Apiou… - Human genetics, 1989 - Springer
E Viegas-Péquignot, LZ Lin, B Dutrillaux, F Apiou, D Paulin
Human genetics, 1989•SpringerA 3-kb DNA fragment, inserted in Bluescribe vector, was used to localize the desmin gene
by in situ hybridization on human metaphase chromosomes. The probe was labelled by Bio-
11-dUTP and detected by immunofluorescence. Subsequent R-banding indicated that the
desmin gene is located in band 2q35.
by in situ hybridization on human metaphase chromosomes. The probe was labelled by Bio-
11-dUTP and detected by immunofluorescence. Subsequent R-banding indicated that the
desmin gene is located in band 2q35.
Summary
A 3-kb DNA fragment, inserted in Bluescribe vector, was used to localize the desmin gene by in situ hybridization on human metaphase chromosomes. The probe was labelled by Bio-11-dUTP and detected by immunofluorescence. Subsequent R-banding indicated that the desmin gene is located in band 2q35.
Springer