[HTML][HTML] Identification of galectin-3-binding protein as a factor secreted by tumor cells that stimulates interleukin-6 expression in the bone marrow stroma
Y Fukaya, H Shimada, LC Wang, E Zandi… - Journal of Biological …, 2008 - ASBMB
We have previously demonstrated that neuroblastoma cells increase the expression of
interleukin-6 by bone marrow stromal cells and that stimulation does not require cell-cell
contact. In this study we report the purification and identification of a protein secreted by
neuroblastoma cells that stimulates interleukin-6 production by stromal cells. Using a series
of chromatographic purification steps including heparin-affinity, ion exchange, and
molecular sieve chromatography followed by trypsin digestion and liquid chromatography …
interleukin-6 by bone marrow stromal cells and that stimulation does not require cell-cell
contact. In this study we report the purification and identification of a protein secreted by
neuroblastoma cells that stimulates interleukin-6 production by stromal cells. Using a series
of chromatographic purification steps including heparin-affinity, ion exchange, and
molecular sieve chromatography followed by trypsin digestion and liquid chromatography …