Purification of recombinant midkine and examination of its biological activities: functional comparison of new heparin binding factors
H Muramatsu, T Muramatsu - Biochemical and biophysical research …, 1991 - Elsevier
H Muramatsu, T Muramatsu
Biochemical and biophysical research communications, 1991•ElsevierMidkine (MK) is the product of a retinoic acid responsive gene, MK, and is the initial member
of a new family of heparin binding factors. Recombinant MK produced by an L-cell clone
transfected with an MK cDNA was purified to homogeneity. When rat embryonic brain cells
were cultured in a serum-free medium on plastic dishes coated with purified MK, a number
of neurites extended, formed networks and fasciculated. The majority of neurons continued
to extend neurites for a week. In control cultures, neurite extension was observed only in a …
of a new family of heparin binding factors. Recombinant MK produced by an L-cell clone
transfected with an MK cDNA was purified to homogeneity. When rat embryonic brain cells
were cultured in a serum-free medium on plastic dishes coated with purified MK, a number
of neurites extended, formed networks and fasciculated. The majority of neurons continued
to extend neurites for a week. In control cultures, neurite extension was observed only in a …
Midkine (MK) is the product of a retinoic acid responsive gene, MK, and is the initial member of a new family of heparin binding factors. Recombinant MK produced by an L-cell clone transfected with an MK cDNA was purified to homogeneity. When rat embryonic brain cells were cultured in a serum-free medium on plastic dishes coated with purified MK, a number of neurites extended, formed networks and fasciculated. The majority of neurons continued to extend neurites for a week. In control cultures, neurite extension was observed only in a few cells. Neurotrophic activity of retinoic acid may be mediated by MK. MK had weak but significant mitogenic activity to 3T3 fibroblasts but not to NRK cells. Thus, MK and other members of the family share a part of the activities.
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