Can oligomeric T-cell receptor be used as a tool to detect viral peptide epitopes on infected cells?
N Anikeeva, T Mareeva, W Liu, Y Sykulev - Clinical Immunology, 2009 - Elsevier
We have utilized soluble HIV Gag-specific T-cell receptor (TCR) D3 with low affinity and TCR-
like antibody 25-D1. 16 recognizing its natural peptide–MHC (pMHC) ligand with high
affinity to determine how affinity and off-rate of the receptor-pMHC interactions affect the
sensitivity of pMHC detection on the cell surface. We found that with soluble TCR cognate
pMHCs can be detected only at relatively high cell surface densities when the TCR was
oligomerized using either Streptavidin or quantum dot (QD) scaffolds. While the higher …
like antibody 25-D1. 16 recognizing its natural peptide–MHC (pMHC) ligand with high
affinity to determine how affinity and off-rate of the receptor-pMHC interactions affect the
sensitivity of pMHC detection on the cell surface. We found that with soluble TCR cognate
pMHCs can be detected only at relatively high cell surface densities when the TCR was
oligomerized using either Streptavidin or quantum dot (QD) scaffolds. While the higher …