The blood–brain barrier (BBB) is essential for maintaining brain homeostasis and protecting
the brain from toxic substances. Breakdown of this barrier results in severe brain
pathologies, whereas impermeability of the BBB is a major obstacle for drug delivery to the
brain. Despite its importance, our understanding of the maturation and modulation of the
BBB is limited. Zebrafish (Danio rerio) has emerged as a useful model organism for studying
vertebrate development and disease mechanisms, as well as for preclinical drug screening …