[HTML][HTML] Identification of hypoxia-response element in the human endothelial nitric-oxide synthase gene promoter
F Coulet, S Nadaud, M Agrapart, F Soubrier - Journal of Biological …, 2003 - ASBMB
The human endothelial nitric-oxide synthase gene (heNOS) is constitutively expressed in
endothelial cells, and its expression is induced under hypoxia. The goal of this study was to
search for regulatory elements of the endothelial nitric-oxide synthase (eNOS) gene
responsive to hypoxia. Levels of eNOS mRNA, measured by real time reverse transcriptase-
PCR analysis, were increased, and heNOS promoter activity was enhanced by hypoxia as
compared with normoxia control experiments. Promoter truncation followed by footprint …
endothelial cells, and its expression is induced under hypoxia. The goal of this study was to
search for regulatory elements of the endothelial nitric-oxide synthase (eNOS) gene
responsive to hypoxia. Levels of eNOS mRNA, measured by real time reverse transcriptase-
PCR analysis, were increased, and heNOS promoter activity was enhanced by hypoxia as
compared with normoxia control experiments. Promoter truncation followed by footprint …