Mutational inactivation of the p53 gene in the human erythroid leukemic K562 cell line
JC Law, MK Ritke, JC Yalowich, GH Leder, RE Ferrell - Leukemia research, 1993 - Elsevier
The K562 human chronic myelogenous leukemia (CML) cell line has attained widespread
use as a model for studying hematologic malignancy and erythroid differentiation.
Sequencing of the p53 gene in the K562 cell line demonstrated a mutation in exon 5
characterized by a single base insertion (cytosine) between codons 135 and 136. This
frameshift mutation leads to an N-terminal truncated protein of 147 amino acids. Only the
mutated sequence was present suggesting that the normal allele has been lost. Reverse …
use as a model for studying hematologic malignancy and erythroid differentiation.
Sequencing of the p53 gene in the K562 cell line demonstrated a mutation in exon 5
characterized by a single base insertion (cytosine) between codons 135 and 136. This
frameshift mutation leads to an N-terminal truncated protein of 147 amino acids. Only the
mutated sequence was present suggesting that the normal allele has been lost. Reverse …