[HTML][HTML] Decay-accelerating factor (DAF), complement receptor 1 (CR1), and factor H dissociate the complement AP C3 convertase (C3bBb) via sites on the type A …
DE Hourcade, L Mitchell, LA Kuttner-Kondo… - Journal of Biological …, 2002 - ASBMB
The AP C3 convertase, C3bBb (Mg 2+), is subject to irreversible dissociation (decay
acceleration) by three proteins: DAF, CR1, and factor H. We have begun to map the factor B
(fB) sites critical to these interactions. We generated a panel of fB mutations, focusing on the
type A domain because it carries divalent cation and C3b-binding elements. C3bBb
complexes were assembled with the mutants and subjected to decay acceleration. Two
critical fB sites were identified with a structural model. 1) Several mutations centered at …
acceleration) by three proteins: DAF, CR1, and factor H. We have begun to map the factor B
(fB) sites critical to these interactions. We generated a panel of fB mutations, focusing on the
type A domain because it carries divalent cation and C3b-binding elements. C3bBb
complexes were assembled with the mutants and subjected to decay acceleration. Two
critical fB sites were identified with a structural model. 1) Several mutations centered at …