[17] Imaging mitochondrial function in intact cells

MR Duchen, A Surin, J Jacobson - Methods in enzymology, 2003 - Elsevier
Publisher Summary This chapter describes the imaging mitochondrial function in intact cells.
Mitochondria play a central role in cell life and cell death. Mitochondria not only generate
ATP, the major currency used by cells in energy-requiring processes, but also house a
range of synthetic enzymes, including enzymes involved in heme and steroid biosynthesis.
Mitochondria are intimately involved in shaping the spatiotemporal characteristics of
intracellular [Ca 2+] c signaling, and they house several proteins that play a key role in the …