Molecular cloning of human interferon cDNA.
T Taniguchi, Y Fujii-Kuriyama… - Proceedings of the …, 1980 - National Acad Sciences
T Taniguchi, Y Fujii-Kuriyama, M Muramatsu
Proceedings of the National Academy of Sciences, 1980•National Acad SciencesA hybrid plasmid, TpIF319, has been shown to contain the sequence for human fibroblast
interferon mRNA [Taniguchi, T., Sakai, M., Fujii-Kuriyama, Y., Muramatsu, M., Kobayashi, S.
& Sudo, T.(1979) Proc. Jpn. Acad. 55, Ser. B, 464-469]. This conclusion was confirmed by a
hybridization-translation assay, using rabbit globin mRNA and its cDNA-containing plasmid
as a control. Plasmid TpIF319 was used as probe and another recombinant plasmid,
TpIF319-13, whose cDNA insert consists of about 800 base pairs, was isolated. Nucleotide …
interferon mRNA [Taniguchi, T., Sakai, M., Fujii-Kuriyama, Y., Muramatsu, M., Kobayashi, S.
& Sudo, T.(1979) Proc. Jpn. Acad. 55, Ser. B, 464-469]. This conclusion was confirmed by a
hybridization-translation assay, using rabbit globin mRNA and its cDNA-containing plasmid
as a control. Plasmid TpIF319 was used as probe and another recombinant plasmid,
TpIF319-13, whose cDNA insert consists of about 800 base pairs, was isolated. Nucleotide …
A hybrid plasmid, TpIF319, has been shown to contain the sequence for human fibroblast interferon mRNA [Taniguchi, T., Sakai, M., Fujii-Kuriyama, Y., Muramatsu, M., Kobayashi, S. & Sudo, T. (1979) Proc. Jpn. Acad. 55, Ser. B, 464-469]. This conclusion was confirmed by a hybridization-translation assay, using rabbit globin mRNA and its cDNA-containing plasmid as a control. Plasmid TpIF319 was used as probe and another recombinant plasmid, TpIF319-13, whose cDNA insert consists of about 800 base pairs, was isolated. Nucleotide sequence analysis of the cDNA revealed that the DNA in fact codes for human fibroblast interferon.
National Acad Sciences
