[HTML][HTML] Specific binding of ApoA-I, enhanced cholesterol efflux, and altered plasma membrane morphology in cells expressing ABC1
Mutations of the ABC1 transporter have been identified as the defect in Tangier disease,
characterized by low HDL and cholesterol ester accumulation in macrophages. A full-length
mouse ABC1 cDNA was used to investigate the mechanisms of lipid efflux to apoA-I or HDL
in transfected 293 cells. ABC1 expression markedly increased cellular cholesterol and
phospholipid efflux to apoA-I but had only minor effects on lipid efflux to HDL. The increased
lipid efflux appears to involve a direct interaction between apoA-I and ABC1, because ABC1 …
characterized by low HDL and cholesterol ester accumulation in macrophages. A full-length
mouse ABC1 cDNA was used to investigate the mechanisms of lipid efflux to apoA-I or HDL
in transfected 293 cells. ABC1 expression markedly increased cellular cholesterol and
phospholipid efflux to apoA-I but had only minor effects on lipid efflux to HDL. The increased
lipid efflux appears to involve a direct interaction between apoA-I and ABC1, because ABC1 …