[HTML][HTML] Direct evidence for a two-step assembly of ApoB48-containing lipoproteins in the lumen of the smooth endoplasmic reticulum of rabbit enterocytes
IJ Cartwright, JA Higgins - Journal of Biological Chemistry, 2001 - ASBMB
The aim of this study was to investigate the types and characteristics of chylomicron
precursors in the lumen of the secretory compartment of rabbit enterocytes. Luminal contents
were separated into density subfractions in two continuous self-generating gradients of
different density profiles. In enterocytes from rabbits fed a low fat diet, newly synthesized and
immunodetectable apoB48 was only in the subfraction of density similar to high density
lipoprotein (dense particles); the luminal triacylglycerol (TAG) content was low and only in …
precursors in the lumen of the secretory compartment of rabbit enterocytes. Luminal contents
were separated into density subfractions in two continuous self-generating gradients of
different density profiles. In enterocytes from rabbits fed a low fat diet, newly synthesized and
immunodetectable apoB48 was only in the subfraction of density similar to high density
lipoprotein (dense particles); the luminal triacylglycerol (TAG) content was low and only in …