[HTML][HTML] Differential activation of the rat phenylethanolamine N-methyltransferase gene by Sp1 and Egr-1
SN Ebert, DL Wong - Journal of Biological Chemistry, 1995 - ASBMB
The rat phenylethanolamine N-methyltransferase (PNMT) gene contains overlapping
consensus elements for the Sp1 and Egr-1 transcription factors located at− 45 bp and− 165
bp in the PNMT promoter. In the present study, we show that Sp1 and Egr-1 can specifically
bind to these overlapping elements, that this binding appears to be mutually exclusive, and
that binding site occupancy is dependent upon the concentration of each factor and its
binding affinity for each site. Egr-1 binds to the− 165 bp site with relatively high affinity (IC …
consensus elements for the Sp1 and Egr-1 transcription factors located at− 45 bp and− 165
bp in the PNMT promoter. In the present study, we show that Sp1 and Egr-1 can specifically
bind to these overlapping elements, that this binding appears to be mutually exclusive, and
that binding site occupancy is dependent upon the concentration of each factor and its
binding affinity for each site. Egr-1 binds to the− 165 bp site with relatively high affinity (IC …