[HTML][HTML] Naturally occurring capsid substitutions render HIV-1 cyclophilin A independent in human cells and TRIM-cyclophilin-resistant in Owl monkey cells

U Chatterji, MD Bobardt, R Stanfield, RG Ptak… - Journal of Biological …, 2005 - ASBMB
In this study, we asked if a naturally occurring HIV-1 variant exists that circumvents CypA
dependence in human cells. To address this issue, we sought viruses for CypA
independence using Debio-025, a cyclosporine A (CsA) analog that disrupts CypA-capsid
interaction. Surprisingly, viral variants from the Main group replicate even in the presence of
the drug. Sequencing analyses revealed that these viruses encode capsid substitutions
within the CypA-binding site (V86P/H87Q/I91V/M96I). When we introduced these …